Soybean (Glycine max (L.) Merr.) is one of the most important crops in the world. The agronomical importance of soybean has been steadily increasing because it is an important source for protein and vegetable oil for human and animal nutrition. In addition, soybean serves as a valuable renewable agricultural source for industrial products, e.g. lubricating oil, printing ink or biodiesel. Soybean has a large size of genome (1,115 Mbp) with 2n=40 in which a complex genome duplication events were involved.
Full-length cDNA collections are regarded as an important resource for post-genomic research, and have therefore already been performed in many organisms. Several techniques have been established to prepare full-length cDNA enriched libraries from various organisms. In plants, full-length cDNAs have also been collected from Arabidopsis, rice, poplar, wheat, maize and so on. A major advantage of this approach is that the most of clones contain the complete coding sequences as well as the 5’ and 3’ untranslated regions (UTRs). Inclusion of the entire sequence data dramatically facilitates the subsequent sequencing, annotation, and protein expression and other functional assays.
Publication : Umezawa et al. (2008) Sequencing and analysis of approximately 40,000 soybean cDNA clones from a full-length-enriched cDNA library. DNA Res., 15, 333-46 [PubMed]
Full-length cDNA information : Gmax_RIKEN_FL-cDNA.zip (11,319kb)
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